Optimization of Equine Influenza Strain A/equine/Otar/764/07 (H3N8) Cultivation in MDCK Cell Culture
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چکیده
The paper presents the results of a study of cultural characteristics of the strain A/equine/Otar/764/07 (H3N8) of equine influenza virus isolated from sick horse during the outbreak of equine influenza epizooty in the Republic of Kazakhstan. The studies determined the optimal culture conditions for equine influenza virus strain A/equine/Otar/764/07 (H3N8) in MDCK cell culture. The optimal infecting dose of the virus was 0,1 TCE50/cell, temperature and cultivation duration 34 ± 0,5 ° C, 72 h, respectively, and Trypsin concentration in medium 2 mg / ml. Subject to the determined parameters of A/equine/Otar/764/07(H3N8) strain cultivation in MDCK the virus-containing suspension with an infectious titer of at least 7,00 lg TCE50/ml and hemagglutinating titer of 8,0 log2 can be obtained. The results obtained during the strain reproductive characteristics study can be used for the development of vaccines and diagnostic kits.
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